Fig. 3

DN-Akt decreases p-/total EGFR expression levels and promotes ROS generation in chemoresistant NSCLC cells in response to CDDP. Chemoresistant H1299 cells were infected with adeno-DN-Akt or adeno-GFP (control; MOI = 0, 30 and 60; 5 h) and treated with CDDP (10 μM, 24 h). a Protein contents of [P-EGFR (Try 1068), EGFR, P-Akt, Akt, p-p53 (Ser 15), p53 and GAPDH (as loading control)] were detected by Western blot. b Statistical analysis and the quantification of indicated proteins were conducted. c Apoptosis was assessed by Hoechst staining. d H1299 Chemoresistant NSCLC cells were infected with adeno-GFP (control; MOI = 60; 5 h), adeno-DN-Akt (MOI = 60; 5 h), adeno-p53 (MOI = 0.25) alone, or together as indicated. e Statistical analysis and the quantification of indicated proteins were conducted [P-EGFR (Try 1068) and P-Akt]. f Apoptosis was assessed by Hoechst staining. Data were analyzed by two-way ANOVA followed by Bonferroni post hoc test (*P < 0.05, ***P < 0.001; CTL vs. CDDP; ^#P < 0.05, ^##P < 0.01, ^####P < 0.0001; CTL vs. Adv-DN-AKT or Adv-p53)