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Fig. 4 | Cancer Cell International

Fig. 4

From: p53 sensitizes chemoresistant non-small cell lung cancer via elevation of reactive oxygen species and suppression of EGFR/PI3K/AKT signaling

Fig. 4

Activation of Akt down-regulates P-p53 and total p53 contents, up-regulates P-EGFR and EGFR contents and suppresses apoptosis in chemosensitive NSCLC cells. Chemosensitive A549 cells were infected with adeno-AAkt or control adeno-GFP (MOI = 0, 30, 60; 5 h) and cultured with CDDP (10 μM, 24 h). a P-EGFR (Try 1068), EGFR, Akt, P-p53 (Ser 15) and p53, and GAPDH (loading control) protein contents were examined by Western blot. b Quantitation and statistical analysis of protein contents [P-EGFR (Try 1068), EGFR, Akt, P-p53 (Ser 15), p53, and GAPDH] were conducted. c After AAkt overexpression and CDDP treatment, change in Akt content in A549 were determined. d Apoptosis was assessed by Hoechst staining. All data are expressed as mean ± SEM (n = 3); Data were analyzed by 2-way ANOVA and Bonferroni post hoc test (*P < 0.05, **P < 0.01, ***P < 0.001; CTL vs. CDDP)

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