Fig. 5

MIAT regulates LASP1 expression via miR-324-3p in papillary thyroid cancer cells. a There was a putative binding site for miR-324-3p on the 3′UTR of LASP1 mRNA. b Overexpression of miR-324-3p reduced relative luciferase activity of LASP1 3′UTR-WT not LASP1 3′UTR-MUT in HTH83 cells. c Overexpression of miR-324-3p reduced relative luciferase activity of LASP1 3′UTR-WT not LASP1 3′UTR-MUT in BHT101 cells. d Transfection of miR-324-3p inhibitor decreased miR-324-3p levels in HTH83 cells. e Downregulation of miR-324-3p increased mRNA levels of LASP1 in both HTH83 and BHT101 cells. f Western blotting showed that downregulation of miR-324-3p increased protein levels of LASP1 in both HTH83 and BHT101 cells. g Quantitative analysis of protein expression in f. h Western blotting showed that silencing of MIAT decreased LASP1 expression which was reversed towards downregulation of miR-324-3p in both HTH83 and BHT101 cells. i Quantitative analysis of protein expression in h. **p < 0.01; ***p < 0.001