Fig. 3

K−MN- and K+MN-bearing cells incline to produce special abnormal chromosome in mitosis with different frequencies. Nuclear and cell divisions with chromosomal segregation anomalies were followed to determine that the relationship between K−MN or K+MN and abnormal chromosome in mitosis of MN-bearing cells. Chromosome fragments carried only H2B-mCherry signals, but no CENP B-GFP. K+LCs emerged in anaphase and carried both H2B-mCherry and CENP B-GFP signals. CBs were identified using H2B-mCherry signals, regardless of the CENP B-GFP status. K−MN-bearing cells produced chromosome fragments much more than MN-free cells in mitosis, while K+MN-bearing cells produced significantly K+LCs more than MN-free cells (*p < 0.05, **p < 0.01, compared with MN-free cells. n = 3)