Fig. 4

miR-125b-5p targets TXNRD1. a, b qRT-PCR and c, d western blot analyses of TXNRD1 levels in HCC cells transfected with the miR-125b-5p mimic or inhibitor (n = 3; Student’s t test). e Diagram of the putative miR-125b-5p-binding site in the TXNRD1 3′ UTR. f Luciferase-reporter assay verifying miR-125b-5p targeting of TXNRD1 in HEK293T cells (n = 3; Student’s t test). g TXNRD1 levels in HCC and non-tumor samples from the TCGA cohort (P < 0.001; Student’s t test). h TXNRD1 expression was inversely correlated with miR-125b levels according to data from HCC patients in the TCGA cohort (P = 0.001; Pearson correlation). i TXNRD1 expression was associated with overall survival of HCC patients according to analysis of the TCGA cohort (P = 0.022; Kaplan–Meier analysis). *P < 0.05; **P < 0.01; ***P < 0.001