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Fig. 3 | Cancer Cell International

Fig. 3

From: LPAR1 regulates the development of intratumoral heterogeneity in ovarian serous cystadenocarcinoma by activating the PI3K/AKT signaling pathway

Fig. 3

Role of LPAR1 in modulating biological functions and the correlation between LPAR1 expression and the PI3K/AKT pathway. a Transwell invasion/migration assays revealed significant decreases in the invasion and migration of the LPAR1 knockdown groups compared with the corresponding control groups (invasion: A2780: 732,395 ± 39,154 vs 467,818 ± 36,623, P = 0.008; SKOV3: 894,327 ± 45,417 vs 537,955 ± 25,284, P = 0.002) (migration: A2780: 598,600 ± 21,514 vs 219,396 ± 13,421, P < 0.001; SKOV3: 711,429 ± 29,264 vs 344,107 ± 20,447, P < 0.001). Similarly, the invasion/migration of the LPAR1-overexpressing cells were significantly increased compared with the corresponding control groups (invasion: A2780: 265,436 ± 19,202 vs 450,506 ± 31,967, P = 0.008, SKOV3: 362,365 ± 22,484 vs 567,782 ± 47,373, P = 0.017) (migration: A2780: 152,081 ± 33,230 vs 468,313 ± 22,950, P = 0.001; SKOV3: 302,498 ± 20,021 vs 497,588 ± 54,052, P = 0.028). b A cell proliferation assay was performed using a CCK-8 kit, and the cell growth curves showed that the LPAR1 deficiency inhibited the proliferation of the A-LV-shLPAR1 and S-LV-shLPAR1 cells (48 h: P = 0.040/< 0.001; 72 h: P = 0.005/0.004). In contrast, LPAR1 overexpression accelerated the proliferation of the A-LV-LPAR1 and S-LV-LPAR1 cells (48 h: P = 0.001/0.002; 72 h: P = 0.039/0.004). In addition, differences in the invasion, migration, and proliferation were not observed between the control groups and the corresponding wild-type (WT) groups (all P > 0.05). c Western blot analyses revealed significantly decreased levels of PI3K p85 alpha phosphorylated at Y607 (p-PI3K Y607) and AKT1/2/3 phosphorylated at S472 + S473 + S474 (p-AKT S472/473/474) in the LPAR1 knockdown cells (p-PI3K: P = 0.003/0.009; p-AKT: P = 0.040/0.010) and significantly increased levels in the LPAR1-overexpressing cells (p-PI3K: P = 0.003/0.016; p-AKT: P = 0.017/0.006). In addition, no differences were observed between the control groups and the corresponding wild-type groups (all P > 0.05)

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