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Fig. 3 | Cancer Cell International

Fig. 3

From: Inhibition LC3B can increase chemosensitivity of ovarian cancer cells

Fig. 3

Validation of candidate miRNAs targeting LC3B in EOC. a Schematic diagram indicating the wild-type and mutated binding sites of miR-204 in the LC3B 3′-UTR. b Co-transfection of miR-204 reduced LC3B luciferase activity in HEK-293TN cells compared with the miR-negative control, whereas this miRNA had no effect on the mutant LC3B 3′-UTR, ***p < 0.001. c, d Western blot and statistical analysis of LC3B and Atg7 expression in OVCAR3 and A2780. LC3B and Atg7 expression were normalized to that of GAPDH. *p < 0.05, **p < 0.01. e, f Western blotting. The LC3B protein in OVCAR3/A2780 cells after transfection. In the presence of 30 μM and 80 μM cisplatin, expression of LC3B was significantly decreased/increased in the miR-204 mimic/inhibitor group compared to the miR-negative group. Protein expression was normalized to that of GAPDH. *p < 0.05, **p < 0.01, ***p < 0.001. g Immunofluorescence staining. Transfection of OVCAR3/A2780 cells with miR-204 mimic/inhibitor or miR-negative. LC3B staining was green, LAMP2 staining was red. Scale bars: 10 µm. h Electron microscopy. Organelle changes in OVCAR3 cells after transfection of miR-204 mimic, inhibitor or miR-negative. The scale bar represents 2 μm. The arrow represents mitochondrion, the triangle-arrow represents lipid droplets, and the star represents autophagic vacuoles

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