Fig. 4

miR-204 enhances cisplatin-induced cytotoxicity in the ovarian cancer cells. a, b Cell viability was assessed by CCK-8 after treatment of A2780 and OVCAR3 cells with cisplatin alone or in combination with miR-204 inhibitor/mimic or miR-negative transfection. c, d Western blotting. Transfection with the miR-204 inhibitor/mimic increased/decreased cleaved-caspase 3, caspase 6 and caspase 9 expression in A2780/OVCAR3 cells. Protein expression was normalized to that of GAPDH. e, f Western blotting. Transfection with the miR-204 inhibitor/mimic increased/decreased p-Bad, Bax, Bak, Bcl-2, cleaved-PARP, Akt and p-Akt expression in A2780/OVCAR3 cells. Protein expression was normalized to that of GAPDH. g Western blotting. Transfection with the miR-204 mimic/inhibitor decreased/increased MDR1 expression in OVCAR3/A2780 cells. MDR1 expression was normalized to that of GAPDH. h qRT-PCR assay. Transfection with the miR-204 mimic/inhibitor decreased/increased MDR1 mRNA expression in OVCAR3/A2780 cells, *p < 0.05. i, j TUNEL staining. Transfection of A2780/OVCAR3 cells with the miR-204 inhibitor/mimic decreased/increased apoptosis compared with the miR-negative group. Apoptosis cells (green), DAPI-stained nuclei (blue). Scale bars: 10 µm