Fig. 3

DIO3OS acts as a molecular sponge for miR-122. a The predicted binding site of miR-122 to the DIO3OS sequence. b Relative expression of miR-122 in MIA PaCa-2 cells after DIO3OS knockdown and in AsPC-1 cells after overexpression of DIO3OS. c Luciferase reporter gene assays were used to confirm the direct binding between miR-122 and DIO3OS. d Lysates of MIA PaCa-2 cells underwent RIP assay with the Ago2 antibody. Expression of DIO3OS and miR-122 was detected using qRT-PCR analysis. Results are shown as fold enrichment of Ago2 relative to IgG immunoprecipitates. e DIO3OS siRNA or control siRNA was transfected into MIA PaCa-2 cells, together with (or without) miR-122 inhibitor. The cells were assayed for cell proliferation (left) and invasion (right). f DIO3OS vector or control vector was transfected into AsPC-1 cells, together with (or without) miR-122 mimic. The cells were assayed for cell proliferation (left) and invasion (right). *P < 0.05