Fig. 4

DIO3OS increases ALDOA expression via inhibiting miR-122 expression. a Predicted miR-122 binding site in the 3′-UTR of ALDOA. b MIA PaCa-2 cells (left) were transfected with luciferase reporter vectors containing wild-type (WT) ALDOA or mutant (MUT) ALDOA, together with (or without) miR-122 mimic, and AsPC-1 cells (right) were transfected with luciferase reporter vectors containing wild-type ALDOA or mutant ALDOA, together with (or without) miR-122 inhibitor. Luciferase reporter gene assays were used to evaluate the interaction between miR-122 and ALDOA. c The protein expression of ALDOA in MIA PaCa-2 cells transfected with miR-122 mimic or control mimic, and in AsPC-1 cells transfected with miR-122 inhibitor or control inhibitor. d The protein expression of ALDOA in MIA PaCa-2 cells transfected with DIO3OS siRNA or control siRNA, together with (or without) miR-122 inhibitor, and in AsPC-1 cells transfected with DIO3OS vector or control vector, together with (or without) miR-122 mimic. The cropped blots are used in the figure. *P < 0.05