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Fig. 3 | Cancer Cell International

Fig. 3

From: Upregulation of circFLNA contributes to laryngeal squamous cell carcinoma migration by circFLNA–miR-486-3p-FLNA axis

Fig. 3

circFLNA promotes cell migration by upregulating FLNA protein expression. a Hep2 cells were transfected pcDNA3.1–circFLNA or pcDNA3.1, shFLNA or negative control, respectively. Western blot analysis was used to detect MMP2 and FLNA protein level. β-actin was used as an internal control. Right panel shows densitometric analysis. **P < 0.01 vs. empty vector. b Hep2 cells were prepared as a, qRT-PCR were used to detect FLNA mRNA expression. c Hep2 cells were transfected shFLNA or negative control. Western blot analysis were used to examine FLNA protein level. Bottom panel shows densitometric analysis. *P < 0.05 vs. empty vector. d Hep2 cells were transfected with pcDNA3.1-circFLNA or sh-FLNA, respectively, or co-transfected with both together. Cell migration was detected by transwell assay. Bottom panel shows migration cell number. *P < 0.05, **P < 0.01 vs. corresponding control

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