Fig. 4

circFLNA functions as a sponge of miR-486-3p in LSCC cell. a Venn diagram hybrid of potential binding miRNAs from three different target prediction programs. b Hep2 cells were transfected with pcDNA3.1-circFLNA or empty vector, pulled down from Hep2 cell lysates with biotin-labeled circFLNA or Ctl probe, qRT-PCR detected pulldown efficiency. *P < 0.05 vs. corresponding control. c The miRNAs were pulled down from Hep2 cell lysates with biotin-labeled circFLNA or Ctl probe. qRT-PCR detected the relative expression of indicated miRNAs. Normalized to U6. *P < 0.05, **P < 0.01 vs. NC probe. d Luciferase reporter assays were performed in Hep2 cells co-transfected with wild-type (WT) or mutant (mut) circFLNA-luciferase reporter and miRNA mimics. *P < 0.05 vs. empty vector. e The expression of miR-486-3p in LSCC tissues and adjacent normal tissues was detected by qRT-PCR analysis