Fig. 5

FLNA is a direct target of miR-486-3p. a Potential miR-486-3p binding site in FLNA 3′-UTR. b Hep2 cells were transfected with miR-486-3p mimic, miR-486-3p inhibitor or its correspondence control. qRT-PCR detected the level of miR-486-3p. *P < 0.05, **P < 0.01 vs. corresponding control. c Hep2 cells were co-transfected with miR-486-3p mimic or mimic-NC and pmir-GLO vector containing wild-type or mutated miR-486-3p-binding site (mut) at FLNA3′-UTR. Luciferase reporter assays were performed. *P < 0.05 vs. mimic-NC. d Hep2 cells treated as b, western blot analysis was used to detected FLNA protein level. Bottom panel shows densitometric analysis of three independent experiments. **P < 0.001 vs. corresponding control. e Hep2 cells treated as b, cell migration was detected by transwell assay. Right panel shows migration cell number analysis of three independent experiments. *P < 0.05 vs. corresponding control