Table 1 The sequences of specific primers
Gene name | Primer sequence (5′ to3′) |
|---|---|
MYLK (convergent primer) | Forward: 5′-AGGTGAGGTCCAGTGTCGT-3′ |
Reverse: 5′-AATGTGCTGGTCACGTTT-3′ | |
CircMYLK (divergent primer) | Forward: 5′-CAATCTTCTTATGCGGCGG-3′ |
Reverse: 5′-GTACCGGCGCAGTCAGG-3′ | |
miR-362-3p | Forward: 5′-CAGGGACTGAGGGCAATCGT-3′ |
Reverse: 5′-TTCATCGCGGTCGAGGGCGG-3′ | |
Rab23 | Forward: 5′-GAAAGTGCTTCGAAAGCGAC-3′ |
Reverse: 5′-TCGCCGAAGTACTTGTGGC-3′ | |
GAPDH | Forward: 5′-ATCCACGGGAGAGCGACAT-3′ |
Reverse: 5′-CAGCTGCTTGTAAAGTGGAC-3′ | |
U6 | Forward: 5′-ACAGATCTGTCGGTGTGGCAC-3′ |
Reverse: 5′-GGCCCCGGATTATCCGACATTC-3′ |