Fig. 2

MiR-21 gene expression analysis after constitutive activation of AP-1. Total RNA and cDNA synthesis were obtained from 1 × 10⁵ SiHa and HaCaT cells per well in a six-well plate containing DMEM at 37 °C with 10% FBS in 5% CO₂ after 60 min of non-treatment (NT) or treatment with 10 ng/ml PMA and 50 μM SR11302. The miR-21 gene expression was analyzed by real-time qRT-PCR and relative expression of miR-21 was calculated using the 2^−∆∆Ct method. The RNU44 gene expression was used as control. The data were analyzed by mRNA miR-21/mRNA RNU44 ratio in relative expression units. The values are presented as mean ± SD and P values < 0.05 are indicated with asterisks. The data are representative of three independent experiments