Fig. 3

Analysis of c-Fos and c-Jun protein expression after constitutive activation of AP-1. Total cellular proteins were obtained from 1 × 10⁵ SiHa (a) and HaCaT cells (b) per well in a six-well plate containing DMEM at 37 °C with 10% FBS in 5% CO₂ after 0, 24, 48 and 72 h treated with 10 ng/ml PMA (lanes 1–4) or 50 μM SR11302 (lanes 5–8). The proteins were separated in 12% SDS-PAGE and were transferred to nitrocellulose membranes, which were incubated with each antibody. Similar amount of proteins were analyzed in the immunoblots. The anti-beta-actin antibody was included as control. The immunoblot bands were digitalized and analyzed by densitometer. The data were analyzed by c-Fos/c-Jun/beta-actin fold change in relative expression units (mean ± SE), not statistically significant (ns) and P values < 0.05 are indicated with asterisks. The data are representative of three independent experiments