Fig. 7
From: Transregulation of microRNA miR-21 promoter by AP-1 transcription factor in cervical cancer cells
Functional analysis of miR-21 promoter activity trans-regulation by AP-1 transcription factor. SiHa, HeLa, C-33A and HaCaT cells were transiently transfected in an independent manner with pG0AP1MIR21, pG1AP1MIR21, pG2AP1MIR21 and pG3AP1MIR21 reporter plasmids that contain progressive deletions of AP1D, AP1M and AP1P sequences of miR-21 promoter. After 48 h of transfection, the luciferase activity levels were analyzed in relative luciferase activity units (mean ± SE), not statistically significant (ns) and P values < 0.05 are indicated with asterisks. The pBL2-Basic and pGL2-Control correspond to negative and positive transfection controls, respectively. NT corresponds to non-transfected cells. All transfections were repeated at least three times independently