Fig. 4

LINC00485 binds to miR-195 thereby up-regulating CHEK1, Bcl-2, VEGF and HIF-1α expression, and down-regulating Bax expression in LAC cells. a miR-195 and LINC00485 expression and the mRNA expression of CHEK1 in A549 cells after treatment of different LINC00485 expression examined by RT-qPCR, GAPDH and U6 as the internal references; b, c the protein bands and expression of CHEK1, Bax, Bcl-2, VEGF and HIF-1α in A549 cells with LINC00485 intervention examined by western blot analysis; d miR-195 and LINC00485 expression and the mRNA expression of CHEK1 in A549 cells after treatment of different miR-195 expression examined by RT-qPCR, GAPDH and U6 as the internal references; e, f the protein bands and expression of CHEK1, Bax, Bcl-2, VEGF and HIF-1α in A549 cells with miR-195 intervention examined by western blot analysis; g miR-195 expression and the mRNA expression of LINC00485 and CHEK1 in H1299 cells after treatment of different LINC00485 expression examined by RT-qPCR, GAPDH and U6 as the internal references; h, i the protein bands and expression of CHEK1, Bax, Bcl-2, VEGF and HIF-1α in H1299 cells with LINC00485 intervention examined by western blot analysis; j miR-195 and LINC00485 expression and the mRNA expression of CHEK1 in H1299 cells after treatment of different miR-195 expression examined by RT-qPCR, GAPDH and U6 as the internal references; k, l the protein bands and expression of CHEK1, Bax, Bcl-2, VEGF and HIF-1α in H1299 cells with miR-195 intervention examined by western blot analysis; *p < 0.05 vs. the control group; ^#p < 0.05 vs. the vector, the si-NC groups or the NC, anta-Ctrl groups. Differences among multiple groups were analyzed by one-way ANOVA. All the experiments were detected 3 times independently, and the results were expressed as mean ± standard deviation. CHEK1, checkpoint kinase 1; Bax, Bcl-2-associated X protein; Bcl-2, B-cell CLL/lymphoma 2; VEGF, vascular endothelial growth factor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase