Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: Interaction between 17β-estradiol, prolactin and human papillomavirus induce E6/E7 transcript and modulate the expression and localization of hormonal receptors

Fig. 2

Expression and location of ERα in HaCaT cells transduced with E6 or E7 oncogenes from HPV-16 and 18. a Relative expression of ERα mRNA was measured by qPCR, the fold change was calculated by 2−ΔΔcq method using ACTB as control. b, c ERα protein expression was determined by western blot, densitometric analysis was performed using β-actin as control. d Immunofluorescence was performed using a secondary antibody conjugated with Alexa Fluor 488 (Green) and DAPI stain (blue). Merged images are shown in magnification 40×. e The location of ERα was observed in cytoplasm and nuclei in immunofluorescence images (*p < 0.05; **p < 0.01; ***p < 0.001)

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us