Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: Interaction between 17β-estradiol, prolactin and human papillomavirus induce E6/E7 transcript and modulate the expression and localization of hormonal receptors

Fig. 3

Expression and location of ERβ in HaCaT cells transduced with the E6 or E7 oncogenes from HPV-16 and 18. a Relative expression of ERβ mRNA was measured by qPCR, the fold change was calculated by 2−ΔΔcq method using ACTB as control. b, c ERβ protein expression was determined with western blot, densitometric analysis was performed using β-actin as control. d immunofluorescence was done using a secondary antibody conjugated with Alexa Fluor 488 (Green) and DAPI stain (blue). Merged images are shown in magnification 40×. e The location of ERβ was observed in cytoplasm and nuclei in immunofluorescence images (*p < 0.05; **p < 0.01; ***p < 0.001)

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us