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Fig. 2 | Cancer Cell International

Fig. 2

From: Haploinsufficiency of NR3C1 drives glucocorticoid resistance in adult acute lymphoblastic leukemia cells by down-regulating the mitochondrial apoptosis axis, and is sensitive to Bcl-2 blockage

Fig. 2

Effects of ectopic expression of NR3C1 in glucocorticoid-resistant ALL cells. a Reh and Jurkat ALL cells were transfected with pLenti-C-Myc-DDK-vector (control), pLenti-C-Myc-DDK-NR3C1. After 5 days, cells were then harvested and analyzed by western blotting with anti-NR3C1 or β-actin antibody. b Results of cell viability assays in transfected cells treated with 1 μmol/l dexamethasone for 48 h. c Results of apoptosis assays in transfected cells treated with 1 μmol/l dexamethasone for 48 h. d Results of leukemic infiltration of spleen, and overall survival in immunodeficient B-NSG mice injected with Reh cells overexpressing the NR3C1 gene or Reh cells transfected with the empty vector. *p < 0.05, **p < 0.01, ***p < 0.001

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