Fig. 3

Effects of deletion of NR3C1 in glucocorticoid-sensitive ALL cells. a CCRF-CEM, 6T-CEM and NALM6 ALL cells were knocked down the NR3C1 gene by CRISPR guide RNA vector transfection. Positive single clones (CCRF-CEM-KO, 6T-CEM-KO and NALM6-KO) were chosen and confirmed by western blotting analysis with anti-NR3C1 or β-actin antibody. b Results of cell viability assays in wild type ALL cells and NR3C1 knockdown cells treated with 1 μmol/l dexamethasone for 48 h. c Results of apoptosis assays in wild type ALL cells and NR3C1 knockdown cells treated with 1 μmol/l dexamethasone for 48 h. d Results of leukemic infiltration of spleen, and overall survival in immunodeficient B-NSG mice injected with NALM6 cells with NR3C1 gene knockdown or wild type NALM6 cells. *p < 0.05, **p < 0.01, ***p < 0.001