Table 1 The primers used for real-time PCR analysis
Genes | Primer sequences |
|---|---|
Human SLPI | Forward 5′-GAGATGTTGTCCTGACACTTGTG-3′ |
Reverse 5′-AGGCTTCCTCCTTGTTGGGT-3′ | |
HPV 16 E6 | Forward 5′-GAGAACTGCAATGTTTCAGGACC-3′ |
Reverse 5′-TGTATAGTTTGCAGCTCTGTGC-3′ | |
Human IL-6 | Forward 5′-ACTCACCTCTTCAGAACGAATTG-3′ |
Reverse 5′-CCATCTTTGGAAGGTTCAGGTTG-3′ | |
Human IL-8 | Forward 5′-TTTTGCCAAGGAGTGCTAAAGA-3′ |
Reverse 5′-AACCCTCTGCACCCAGTTTTC-3′ | |
Human IL-1β | Forward 5′-ATGATGGCTTATTACAGTGGCAA-3′ |
Reverse 5′-GTCGGAGATTCGTAGCTGGA-3′ | |
Human TNF-α | Forward 5′-CCTCTCTCTAATCAGCCCTCTG-3′ |
Reverse 5′-GAGGACCTGGGAGTAGATGAG-3′ | |
Human c-myc | Forward 5′-GGCTCCTGGCAAAAGGTCA-3′ |
Reverse 5′-CTGCGTAGTTGTGCTGATCA-3′ | |
Human GAPDH | Forward 5′-ACAACTTTGGTATCGTGGAAGG-3′ |
Reverse 5′-GCCATCACGCCACAGTTTC-3′ |