Fig. 1From: miR-145 sensitizes esophageal squamous cell carcinoma to cisplatin through directly inhibiting PI3K/AKT signaling pathwayThe expression level of miR-145 and AKT3 in ESCC tissues and cells. The level of miR-145 (a) and AKT3 (b) in ESCC tissue (n = 30) compared with adjacent normal tissues was detected by qRT-PCR. The expression level of miR-145 (c) and AKT3 (d) was detected by qRT-PCR in normal esophageal squamous cells line and ESCC cell lines. e Western blot analysis of AKT3 and p-AKT in normal esophageal squamous cells line and ESCC cell lines. f Quantification of relative protein level for Western blotting. Total 30 subjects were analyzed. All the results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. *p < 0.05 and **p < 0.01Back to article page