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Fig. 7 | Cancer Cell International

Fig. 7

From: miR-145 sensitizes esophageal squamous cell carcinoma to cisplatin through directly inhibiting PI3K/AKT signaling pathway

Fig. 7

Knockdown of miR-145 inhibited the sensitivity of ESCC cells to DDP by targeting AKT3. The expression level of miR-145 (a) and AKT3 (b) was detected by qRT-PCR in EC9706 cells transfected with miR-145 inhibitor or miR-NC. c The multidrug resistance and cell proliferation-related protein level was detected by Western blotting in EC9706 cells transfected with miR-145 inhibitor, pcDNA3.1-AKT3 or sh-AKT3. d Cell viability was detected by MTT assay in EC9706 cells exposed to DDP after transfection with miR-145 inhibitor, pcDNA3.1-AKT3 or sh-AKT3. e Analysis and quantifications of DDP IC50 value. f Cell apoptosis was analyzed with flow cytometry in EC9706 cells exposed to DDP after transfection with miR-145 inhibitor, pcDNA3.1-AKT3 or sh-AKT3. g Apoptosis cells were quantified. All the results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. *p < 0.05 and **p < 0.01

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