Fig. 4

XPB and Ki67 immunoexpressions and pathologic features in human colon cancer specimens. a Single tissue section presenting two distinct regions: a malignant region (ROI 1) and an adjacent normal colon mucosa (ROI 2). Elevated XPB and Ki67 expression appears frequently in the tumor cells of a colon cancer tissue (ROI1) compared to the cells of the adjacent normal mucosa (ROI 2). In the normal colon mucosa, a few cells showing concomitant high expression of XPB and Ki67 are seen in the deeper regions of colonic crypts where proliferating cells are expected to be present. b, c Zoomed in ROI 1 and 2. Proliferating cells present high levels of both XPB (green) and Ki67 (red) and are easily identified by their yellow nuclei in both merged images. d, e Consecutive serial section stained with hematoxylin to evaluate the morphology of the tissue. All scale bars represent 100 µm