Fig. 3

NAP1L1 knockdown suppresses tumor growth and increasing chemosensitivity in vitro. a Cell growth of 7721-shNAP1L1, sk-hep-1-shNAP1L1 and the control cells was analyzed by CCK8 assays. b Cytotoxicity assays of 7721-shNAP1L1, SK-hep-1-shNAP1L1 and the control cells was analyzed by CCK8 assays. c Flow cytometry analysis 7721-shNAP1L1, sk-hep-1-shNAP1L1 and the control cells treated with DOX for 24 h. d Cancer stem related markers’ mRNA expression levels were measured in the 7721-shNAP1L1, sk-hep-1-shNAP1L1 and the control cells. e Western blotting analyzed NAP1L1 protein levels in different cell lines (left upper). Western blotting showed NAP1L1 significantly downregulated in 7721-shNAP1L1, sk-hep-1-shNAP1L1 compared to their respective control cells (left down). Western blotting analyzed NOTCH1, ABCG2, C-MYC, OCT4 and SOX2 protein level in 7721-shNAP1L1, sk-hep-1-shNAP1L1 and their respective control cells (right). Abbreviations: DOX, doxorubicin; p < 0.05, **p < 0.01, ***p < 0.001