Fig. 2

SNHG6 enhances autophagy in CRC cells and 5FU-resistance in vivo. a Western blot analysis showed SNHG6 could increase level of LC3-II, an autophagy-related protein, in CRC cell. b–e Relative expression of LC3B proteins. f Immunofluorescence RKO-shSNHG6 cells had fewer numbers of autophagosome. g, h RKO-shSNHG6 cells reduced tumor growth in vivo and promoted tumor sensitivity to 5-FU. i RKO-shSNHG6 cells had a higher tumor growth inhibition ratio with 5-FU treatment than control cells did. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, data was shown as the mean ± SD