Fig. 2

UCA1 up-regulates the expression of PTP1B in breast cancer cells. a MCF-7 and MDA-MB-231 cells were transfected with UCA1 or vector using Lipofectamine 2000 reagent. RNA was extracted 24 h after transfection. The mRNA levels of PTP1B was measured by RT-PCR analysis. b, c MCF-7 and MDA-MB-231 cells were transfected with UCA1 or vector using Lipofectamine 2000 reagent. Protein was extracted 48 h after transfection. The protein levels of PTP1B were examined by Western blot analysis. d MCF-7 and MDA-MB-231 were transfected with siRNA-UCA1 or siRNA-control using Lipofectamine 2000 reagent. RNA was extracted 24 h after transfection. The mRNA levels of PTP1B was tested by RT-PCR analysis. e, f MCF-7 and MDA-MB-231cells were transfected with siRNA-UCA1 or siRNA-control using Lipofectamine 2000 reagent. Protein was extracted 48 h after transfection. The protein levels of PTP1B was evaluated by Western blot analysis. Statistical significant differences are indicated: **p < 0.01, Student’s t-test