Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: Ethanol extract of Ligustrum lucidum Ait. leaves suppressed hepatocellular carcinoma in vitro and in vivo

Fig. 2

Effect of EEL treatment on the expression levels of apoptosis-related genes. Bel-7402 and Huh-7 cells in control, EEL1, EEL2 and EEL3 groups were respectively treated with 0, 25, 50 and 75 mg/ml EEL for 24 h. Data in ae were generated with Bel-7402 cells, while data in F-J were produced with Huh-7 cells. ac mRNA levels of Bax, Bcl-2 and Cyt-C in Bel-7402 were determined using RT-qPCR. d Protein expression of Bax, Bcl-2 and Cyt-C in Bel-7402 were analyzed using Western blotting assay. e The expression of active caspase-3 was detected by IHC. fh mRNA levels of Bax, Bcl-2 and Cyt-C in Huh-7 were determined using RT-qPCR. i Protein expressions of Bax, Bcl-2 and Cyt-C in Bel-7402 were determined using Western blotting assay. j The expression of active caspase-3 was detected by IHC. Data were shown as mean ± S.D. for three independent experiments. *P < 0.05, **P < 0.01 versus control

Back to article page