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Fig. 4 | Cancer Cell International

Fig. 4

From: Ethanol extract of Ligustrum lucidum Ait. leaves suppressed hepatocellular carcinoma in vitro and in vivo

Fig. 4

The effects of EEL treatment on the expression levels of TIMP2, MMP2 and MMP9. Bel-7402 and Huh-7 cells in control, EEL1, EEL2 and EEL3 groups were treated with respectively 0, 25, 50 and 75 mg/ml EEL for 24 h. ac mRNA levels of TIM2, MMP2 and MMP9 in Bel-7402 were determined using RT-qPCR. d Protein expression of TIM2, MMP2 and MMP9 in Bel-7402 were analyzed using Western blotting assay. eg mRNA levels of TIM2, MMP2 and MMP9 in Huh-7 were determined using RT-qPCR. h Protein expression of TIM2, MMP2 and MMP9 in Huh-7 were determined using Western blotting assay. Data were shown as mean ± S.D. for three independent experiments. *P < 0.05, **P < 0.01 versus control

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