Fig. 5

Rac1 is required for TUFT1-mediated NF-κB pathway and its downstream factors activation in TNBC cells. a TUFT1 expression was downregulated by TUFT1-shRNA and upregulated by PLNCX2-TUFT1 in MDA-MB-231 and HCC1937 cells. Protein levels of p-p65, p-65, XIAP, Survivin, cleaved caspase 3 and cleaved PARP1 were examined by western blotting (n = 3). b TUFT1-overexpressing MDA-MB-231 and HCC1937 cells treated with or without NSC23766. Protein levels of p-p65, p-65, XIAP, Survivin, cleaved caspase 3 and cleaved PARP1 were examined by western blotting (n = 3). Results are presented as mean ± SD. The statistical significance was assessed by student’s t-test; **p < 0.01