Fig. 1

MiR-141 suppresses the expression of NRP-1 in pancreatic cancer cells by binding to the 3′UTR of NRP-1 gene. a Predicted binding site of Hsa-miR-141 to the 3′-UTR of the human NRP-1 gene, and the diagram of a pMIR-REPORT luciferase reporter vectors containing the wild-type (WT) or mutated (MT) 3′-UTR of NRP-1. b BxPC-3 cells were transfected with the WT luciferase reporter and co-transfected with negative control oligonucleotides (NC) or various miRNA mimics as indicated. Mock-transfected cells served as a control. c The potential binding sites of miR-141 (underlined) in the 3′UTR region of NRP-1 is highly conserved across species. d BxPC-3 cells were transfected with the WT luciferase reporter and co-transfected with different concentrations of miR-141 mimics. e BxPC-3 cells were transfected with WT or MT luciferase reporters and co-transfected with NC, miR-141 mimics or anti-miR-141. Relative luciferase activity was calculated as the percentage of luciferase activity in luciferase reporter-transfected cells over the control. “ϕϕ” (P < 0.001) indicates a significant reduction, while “##” (P < 0.001), a significant increase, compared with NC-transfected cells. “**” (P < 0.001) indicates a significant difference