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Fig. 4 | Cancer Cell International

Fig. 4

From: The miR-141/neuropilin-1 axis is associated with the clinicopathology and contributes to the growth and metastasis of pancreatic cancer

Fig. 4

NRP-1 promotes the migration of pancreatic cancer cells by regulating EMT. a, b BxPC-3 and BxPC-NRPlow cells were subjected to Transwell migration assays. a Migrated cells were visualized using Giemsa staining. Magnification bar = 200 µm. b Numbers of migrating cells were counted. c, d Cells were subjected to scratch migration assays. Scratch areas were recorded (c) and scratch distances were quantified (d) at indicated time points. e, f Cells were immunoblotted for detecting key EMT proteins (e) and the density of each band was normalized to β-actin (f). g Cells were subjected to gelatin zymography assays for analyzing the gelatinolytic activity of MMP-9 and MMP-2. “**” (P < 0.001) indicates a significant difference from BxPC-3 cells

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