Fig. 6
MiR-141 inhibits cell migration and EMT by dysregulating NRP-1 that co-interacts with TGF-β ligand to activate the TGF-β signaling pathway. a, b BxPC-3 and BxPC-NRPlow cells were transfected with negative control (NC), miR-141 mimics or anti-miR-141 oligonucleotides for 48 h. Cells were then subjected to Transwell migration assays and numbers of migrating cells were recorded (a), or immunoblotting for detecting key EMT proteins (b). c BxPC-3 and BxPC-NRPlow cells were incubated in the presence or absence of recombinant TGF-β protein (5 ng/ml) or LY2157299 (10 μg/ml) for 24 h, and subjected to immunoblotting. The density of each band was normalized to β-actin. “*” (P < 0.05) and “**” (P < 0.001) indicate a significant difference. “ϕ” (P < 0.05) indicates a significant reduction, while “#” (P < 0.05), a significant increase, from Vehicle-treated BxPC-3 cells