Fig. 6

KLF8 promotes the expression of JMJD2A. a, b KLF8 knockdown reduces the expression of JMJD2A. A549 cells were infected with indicated lentivirus for 24 h and then the mRNA (a) and protein (b) level of JMJD2A were analyzed. ** p < 0.01 vs. shCtrl. n = 3 in each group. c, d KLF8 overexpression promotes the expression of JMJD2A. A549 cells were infected with indicated lentivirus for 24 h and then the mRNA (a) and protein (b) level of JMJD2A were analyzed. n = 3 in each group. e Chromatin-immunoprecipitation (ChIP) assay showing KLF8 binding to JMJD2A promoter. **p < 0.01 vs. shCtrl + ChIP-IgG, ^##p < 0.01 vs. shCtrl + ChIP-KLF8. n = 3 in each group. f KLF8 knockdown reduces the promoter activity of JMJD2A. A549 cells with/without shKLF8 transduction were transfected with pGL3-JMJD2A for 24 h, then cells were subjected to luciferase analysis. **p < 0.01 vs. shCtrl. n = 3 in each group. g KLF8 overexpression increases the promoter activity of JMJD2A. A549 cells with/without KLF8 transduction were transfected with pGL3-JMJD2A for 24 h, then cells were subjected to luciferase analysis. **p < 0.01 vs. Lenti-Ctrl. n = 3 in each group