Skip to main content
Fig. 4 | Cancer Cell International

Fig. 4

From: Extracellular and macropinocytosis internalized ATP work together to induce epithelial–mesenchymal transition and other early metastatic activities in lung cancer

Fig. 4

Extracellular ATP induces changes in levels and cellular distribution of proteins involved EMT. a A549 cells were treated with 0, 0.5 or 1.0 mM ATP for 24 h before analysis. EMT- associated proteins expression level was detected using western blot analysis. β-actin was used as a protein loading control for protein normalization. b Densitometry analyses of individual blots were performed using ImageJ software. c Immunofluorescence analysis of EMT-related proteins. Control and 0.5 mM ATP-treated A549 cells were fixed and stained with anti-E-Cadherin and vimentin antibodies observed by fluorescence microscopy with 400× magnification. Experiments were performed in triplicate and data are presented as the mean ± standard deviation. *P < 0.05, **P < 0.01 and ***P < 0.001

Back to article page