Fig. 5

Knockdown of RUVBL2 expression inhibited cell proliferation and survival. a, d Cell proliferation was measured with CCK-8 assay at indicated times after the transfection of specific siRNA duplexes against RUVBL2 in HepG2 (a) and Huh7 (d) cells. The absorbance is shown as the mean ± standard error for each day. b, e The colony formation assay in HepG2 (b) and Huh7 (e) at 24 h after the transfection of specific siRNA duplexes against RUVBL2. Representative dishes are shown in the left panel, and quantitative colony numbers are compared in the right panel. c, f The migration and invasion assays (left panels) were performed in HepG2 (c) and Huh7 (f) at 24 h after the transfection of specific siRNA duplexes against RUVBL2. Cell migration and invasion capability is shown in the right panel by counting cells per field. For (a–f), Scr, scrambled negative control; siR-1, siRUVBL2-1; siR-2, siRUVBL2-2; *P < < 0.05; **P < < 0.01; ***P < < 0.001; ****P < < 0.0001. g, h Western blot analysis of cell proliferation- and survival-associated signaling genes in HepG2 (g) and Huh7 (h) at 24 h post-transfection with specific siRNA duplexes against RUVBL2. Densitometry was performed to quantify each lane, and the ratio of each protein over the loading control β-actin is presented under each blot, with the ratio in the scramble group being the reference value