Fig. 3

LRSAM1 promotes the anchorage-independent growth of human HCC cells. HepG2 single clones stably expressing control shRNA or LRSAM1 shRNAs (a–d) or HepG2 mixed clones stably expressing GFP or GFP-LRSAM1 were generated. The cells were subjected to Western blot analysis for LRSAM1 expression (a, c, e) and soft agar assays to examine the colony forming ability (b, d, f). The data are shown as the mean ± SD, n = 3; *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance