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Table 2 Comparison between cfDNA and short-length exosomal nucleic acids

From: Selecting short length nucleic acids localized in exosomes improves plasma EGFR mutation detection in NSCLC patients

Samplea Target Mutant events Wild-type events Fraction (%) Mutant allele ratio Wild-type allele ratio
exoDNA/cfDNA exoTNA/cfDNA exoDNA/cfDNA exoTNA/cfDNA
Spiked sample 1 (Ex19del) cfDNA 20 418 4.78 0.5 1.2 0.6 2.7
Short-length-exoDNA 9 270 3.33
Short-length-exoTNA 24 1138 2.11
Spiked sample 2 (L858R) cfDNA 1 156 0.64 1 2 0.8 2.6
Short-length-exoDNA 1 131 0.76
Short-length-exoTNA 2 399 0.5
Spiked sample 3 (L858R) cfDNA 2 38 5.26 0 2.5 0 6
Short-length-exoDNA 0 1 0
Short-length-exoTNA 5 228 2.19
Spiked sample 3 (T790M) cfDNA 0 97 0 N.A. N.A. N.A. N.A.
Short-length-exoDNA 6 89 6.74
Short-length-exoTNA 8 307 2.61
  1. cfDNA cell-free DNA, exoDNA exosomal DNA, exoTNA exosomal DNA and RNA, Ex19del exon 19 deletion, N.A not available
  2. aSpiked samples with pooled plasma from patients harboring mutations in EGFR (Ex19del, L858R, and T790M). cfDNA and short-length exoDNA were extracted using MagMAX Cell-Free DNA Isolation Kit. Short-length exoTNA was extracted using MagMAX™ Total Nucleic Acid Isolation Kit