Fig. 4

SUV39H2 knockdown inhibits cancer stem cell properties and promotes apoptosis induced by TMZ in glioma cells. a Sphere formation capacity of CTRL and SUV39H2-sh U251 or U87 cells (scale bar, 200 μm). b mRNA expression of the indicated genes related to stemness was assessed in U251 or U87 cells. c In vivo tumor initiation assay of CTRL and SUV39H2-sh glioma cells. d An annexin-V/7AAD assay was performed to measure cell apoptosis in CTRL and SUV39H2-sh cells in the absence or presence of 100 μM TMZ for 48 h. e γH2A.X formation was measured by IF staining in U251 cells treated with 100 μM TMZ for 24 h. Data are shown as the mean ± SEM of at least three independent experiments. (*p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test)