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Fig. 5 | Cancer Cell International

Fig. 5

From: Histone methyltransferase SUV39H2 regulates cell growth and chemosensitivity in glioma via regulation of hedgehog signaling

Fig. 5

SUV39H2 regulates the properties of cancer stem cells in glioma cells by modulating hedgehog signaling. a RBP-Jκ, GLI, and TCF/LEF1 responsive luciferase reporter assays were performed in CTRL and SUV39H2-sh glioma cells. b Western blot analysis of the indicated proteins related to Hh signaling in U251 and U87 cells. c Correlation between SUV39H2 and HHIP levels in glioma patients (n = 180) from the GSE4290 database (http://r2.amc.nl). d Representative images of SUV39H2 and HHIP IHC staining in the indicated xenograft tumors. e Representative images and cartograms of the colony formation assay in CTRL, SUV39H2-sh, HHIP-sh, and SUV39H2-sh plus HHIP-sh U251 cells. f An annexin-V/7AAD assay was performed to measure cell apoptosis induced by TMZ in CTRL, SUV39H2-sh, HHIP-sh, and SUV39H2-sh plus HHIP-sh U251 cells. Data are shown as the mean ± SEM of at least three independent experiments. (*p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test)

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Cancer Cell International

ISSN: 1475-2867

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