Fig. 5From: Long non-coding RNA HOTAIR functions as a competitive endogenous RNA to regulate PRAF2 expression by sponging miR-326 in cutaneous squamous cell carcinomaPRAF2 is the direct target of miR-326. a The putative miRNA binding sites in the PRAF2 sequence. b Dual-luciferase reporter gene assay is carried out to verify the direct target sites. c PRAF2 expression in CSCC cell lines and HaCaT detected by qRT-PCR. d Protein levels of PRAF2 in HaCaT, A431 and SCL-1 are detected by Western blotting. Data are presented as mean ± SD. *P < 0.05Back to article page