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Fig. 5 | Cancer Cell International

Fig. 5

From: Long non-coding RNA HOTAIR functions as a competitive endogenous RNA to regulate PRAF2 expression by sponging miR-326 in cutaneous squamous cell carcinoma

Fig. 5

PRAF2 is the direct target of miR-326. a The putative miRNA binding sites in the PRAF2 sequence. b Dual-luciferase reporter gene assay is carried out to verify the direct target sites. c PRAF2 expression in CSCC cell lines and HaCaT detected by qRT-PCR. d Protein levels of PRAF2 in HaCaT, A431 and SCL-1 are detected by Western blotting. Data are presented as mean ± SD. *P < 0.05

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