Fig. 6

HOTAIR/miR-326 axis is pivotal for the expression of PRAF2. a MiR-326 inhibitors or/and HOTAIR siRNAs are transfected into A431 and the mRNA level of PRAF2 is measured via qRT-PCR. b Western blotting analysis of PRAF2 protein level following treatment of A431 with miR-326 inhibitors or/andHOTAIR siRNAs, with β-actin as a control. c SCL-1 is transfected with miR-326 or/and HOTAIR OE plasmids and the relative mRNA levels of PRAF2 compared with controls are monitored via qRT-PCR. d Relative protein level of PRAF2 in cells transfected with miR-326 mimics or/and HOTAIR OE plasmids. e Relative mRNA level of PRAF2 in cells transfected with HOTAIR-WT OE plasmids or HOTAIR-MUT OE plasmids. f Relative protein level of PRAF2 transfected with HOTAIR-WT OE plasmids or HOTAIR-MUT OE plasmids. Data are presented as mean ± SD. *P < 0.05 compared with control; ns, no significant difference