Fig. 7

LncRNA-LET is involved in cell growth of ccRCC cells by targeting miR-373-3p. a MiR-373-3p level was assayed in ccRCC cells with miR-373-3p mimics or inhibitor via quantitative real-time PCR. b LncRNA-LET level was assayed in miR-373-3p mimics or inhibitor ccRCC cells via quantitative real-time PCR. c Schematic diagram: lncRNA-LET (black) function as a target of miR-373-3p (green). MiR-373-3p was indicated to bind with DKK1 (red) and TIMP2 (burgandy). ORF was filled with rectangles. d The binding sites between lncRNA-LET and miR-373-3p, miR-373-3p and DKK1, miR-373-3p and TIMP2 were predicted. e The relationship between lncRNA-LET and miR-373-3p was demonstrated through luciferase reporter assay. f DKK1 and TIMP2 protein levels were examined with western blot analysis. ^##p < 0.01, ^###p < 0.001. ccRCC clear cell renal cell carcinoma, DKK1 Dickkopf-1, TIMP2 tissue inhibitor of metalloproteinase-2, mimics NC negative control mimics