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Fig. 5 | Cancer Cell International

Fig. 5

From: The lncRNA DLX6-AS1 promoted cell proliferation, invasion, migration and epithelial-to-mesenchymal transition in bladder cancer via modulating Wnt/β-catenin signaling pathway

Fig. 5

Knockdown of DLX6-AS1 inhibited Wnt/β-catenin signaling in T24 cells. a Analysis of β-catenin, GSK-3β, c-myc and cyclin D1 mRNA expression by qRT-PCR in T24 cells after being transfected with control siRNA or DLX6-AS1 siRNA. b Analysis of β-catenin, GSK-3β, c-myc and cyclin D1 protein expression by western blot in T24 cells after being transfected with control siRNA or DLX6-AS1 siRNA. c Analysis of cell growth by colony formation assay, d analysis of cell proliferation by CCK-8 assay, e analysis of cell invasion by transwell invasion assay and f analysis of cell migration by transwell migration assay in vehicle or LiCl-treated T24 cells after being transfected with control siRNA or DLX6-AS1 siRNA. N = 3; significant differences between different groups were shown as *P < 0.05, **P < 0.01 and ***P < 0.001

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