Table 1 Effects of matrix fixation and NHE1 expression on cell migration and morphology
Matrix | Native | Fixed | ||
|---|---|---|---|---|
NHE1 expression | Mock control (n = 30; N = 5) | Overexpressed (n = 40; N = 3) | Mock control (n = 40; N = 3) | Overexpressed (n = 41; N = 3) |
Speed [µm min−1] | 0.44 ± 0.03 | 0.034 ± 0.02 | 0.48 ± 0.04 | 0.13 ± 0.01 |
Net distance [µm] | 61.92 ± 9.42 | 45.17 ± 5.6 | 57.92 ± 9.07 | 11.93 ± 2.33 |
Total distance [µm] | 130.92 ± 8.61 | 103.12 ± 5.78 | 143.37 ± 10.67 | 37.84 ± 2.83 |
Directionality | 0.45 ± 0.05 | 0.44 ± 0.04 | 0.38 ± 0.04 | 0.28 ± 0.04 |
Structural index | 0.43 ± 0.03 | 0.5 ± 0.02 | 0.3 ± 0.01 | 0.42 ± 0.02 |
Area [µm2] | 888 ± 112 | 1115 ± 84 | 1251 ± 78 | 1298 ± 94 |