Fig. 5

DANCR knockdown repressed tumor cell progression and KLF8 expression by targeting miR-135a-5p in vitro. a The viability of CAL-27 and TCa-8113 cells was measured using MTT assay. b, c The migration and invasion of CAL-27 and TCa-8113 cells were detected by wound healing assay and transwell assay, respectively. d Relative expression of MMP-2 and MMP-9 protein was determined by western blot in CAL-27 and TCa-8113 cells. e Relative expression of KLF8 protein was detected using western blot in CAL-27 and TCa-8113 cells. *p < 0.05, **p < 0.01, ***p < 0.001, versus to si-NC; ^&p < 0.05, ^&&p < 0.01, ^&&&p < 0.001, versus to si-DANCR-1 + NC inhibitor