Fig. 4

MiR-140-5p regulated SACC-83 and SACC-LM cancer cell progression via targeting survivin. a, b CCK-8 assay, c colony formation assay, d transwell invasion assay respectively determined cell proliferation, growth and invasion of SACC-83 and SACC-LM cells after being treated with 100 nM YM-155 or normal medium (NC). e Flow cytometry and f caspase-3 activity assay respectively determine cell apoptotic rates and caspase-3 activity of SACC-83 and SACC-LM cells after being treated with 100 nM YM-155 or normal medium (NC). g qRT-PCR determined survivin mRNA expression levels in SACC-83 and SACC-LM cells after being transfected with pcDNA3.1 or pcDNA3.1-survivin. h, i CCK-8 assay, j colony formation assay, k transwell invasion assay respectively determined cell proliferation, growth and invasion of SACC-83 and SACC-LM cells after being co-transfected with mimics NC + pcDNA3.1, miR-140-5p mimics + pcDNA3.1 or miR-140-5p mimics + pcDNA3.1-survivin. f Flow cytometry and g caspase-3 activity assay respectively determine cell apoptotic rates and caspase-3 activity of SACC-83 and SACC-LM cells after being co-transfected with mimics NC + pcDNA3.1, miR-140-5p mimics + pcDNA3.1 or miR-140-5p mimics + pcDNA3.1-survivin. N = 3. Significant difference between treatment groups were indicated as *P < 0.05 and **P < 0.01