Fig. 2

Effects of abiraterone and MDV3100 on mtDNA, mΔψ and morphology in LNCaP cell. a Detection of mitochondrial DNA copy number. Error bars represent mean ± S.D. of three independent experiments; *p < 0.05, **p < 0.01 versus control, based on unpaired t-test. b, c Mitochondrial membrane potential (mΔψ) detection. The representative dot plots of JC-1 fluorescence in the LNCaP cells treated with 20 nmol/L abiraterone for 24 h or 10 nmol/L MDV3100 for 48 h. 50 M/L CCCP for 5 min working as positive control of the assay. Error bars represent mean ± S.D. of three independent experiments; n ≥ 10,000 cells/experiment. *p < 0.05, **p < 0.01, ***p < 0.001versus control, based on unpaired t-test. d Mitochondrial morphology analysis. Electron micrographs show swelling mitochondria and autophagosomes induced by abiraterone and MDV3100. The white arrows in the electron micrographs represent autophagosomes, the black ones represent swelling mitochondria in abiraterone and MDV3100 treatment groups and represent healthy mitochondria in control group. Scale bar, 500 nm. The experiments were performed three times, and a representative result is shown above